PE Anti-Human TCR Vd2 Antibody

JvGCRC
Nottingham Trent University
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human TCR Vδ2 antibody

Catalog Number:

331408

This antibody has been used in a flow cytometry based study immunophenotyping PBMC populations obtained from cancer patients. The results have not yet been published. A multi-colour antibody panel was designed to identify a number of T cell populations on the basis of TCR expression. The panel was tested to ensure appropriate compensation was possible. This antibody was used to identify subsets of γδ T cell population expressing δ2.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood mononuclear cells (PBMC)

Primary Incubation

2.5uL PE TCR Vδ2 mAb for 30 minutes at 4oC

Blocking Agent

FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Beckman Coulter Galios, Kaluza software

Results Summary

Clear staining that allowed the identification of γδ T cells expressing δ2. This antibody fit well into a ten colour panel with no compensation issues.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Clear staining and useful for FACS analysis.

The Bad

The antibody has only been tested for flow cytometry

The Bottom Line

Easy to use antibody pack that provided clear and distinct staining of a subset of γδ T cells.

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