Biolegend
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) Antibody
118212
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: CD326 Immunofluorescence Antibodies
We study tumorgenesis in the PyMT mouse model of breast cancer. As a first step, we wanted to understand epithelial cell biology under normal mammary gland development. We chose this Ep-CAM antibody due to its reliability.
Immunofluorescence
PLP fixed mouse (C57BL/6) lactating mammary gland cryosections
2.5ug/ml for 3h at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
None
Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 40x 1.3NA air objective.
20um cryosections of lactating mammary gland were stained with anti-Ep-CAM Alexa Fluor® 647 (green) and counterstained with DAPI (blue).
N/A
Reliable & reproducible staining of epithelial cells
Pretty good antibody. Nevertheless, personally I tend to prefer to stain for E-Cadherin rather than Ep-CAM.