Cell Signaling Technology
Phospho-Smad2 (Ser245/250/255)
3104
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We assessed molecular markers with the potential to predict retinoic acid responsiveness of AML blasts. We assessed if phospho-Smad2 (Ser245/250/255) correlates with colony forming capacity of primary leukemia blasts. We selected this product because we had prior good results with antibodies from Cell Signaling Technology.
Western Blot
HL-60 cells ; primary leukemia blasts
Overnight at 4 degrees Celsius
BSA
HRP anti-rabbit IgG, 1h, RT
None
Chemiluminescence
Phospho-Smad2 (Ser245/250/255) antibody (#3104 Cell Signaling ) has high affinity for its target. However it does have some background that would be interesting to be identify (upper band).
10.18632/oncotarget.23642
N/A
Detects the band of interest
Has some background that would be interesting to identify
It is a good antibody