Santa Cruz Biotechnology
Raf-1 Antibody (E-10)
sc-7267
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Browse Similar Products: Raf-1 Western Blot Antibodies
Raf-1 is a Ser/Thr kinases intrinsic kinase activity. Mutation in Raf protein is often observed in several human malignancies. We determined its levels in MCF7 cells after treatment with the drug 1 and drug 2.
Western Blot
Cell Lysates of MCF7
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:10000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We could see the Raf-1 specific bands but over-exposure can cause non-specific background noise.
N/A
Efficacy and binding affinity
Nothing
In conclusion, its a great antibody for Western blotting.