Santa Cruz Biotechnology
Akt1/2/3 Antibody (5C10)
sc-81434
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PI3K/Akt pathway is central to several cellular processes in the cell. Activated Akt is involved in cancer cell development and progression. We determined the expression levels of Total Akt in different cell lines by using this antibody
Western Blot
Cell lysates of Hela, Caski, SiHa
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We obtained a single thick band specific to Akt. The antibody worked really well.
N/A
Specificity and reactivity
Nothing
Overall, its a good antibody