Alpha-tubulin Antibody for Western Blotting Normalization

February 27, 2018

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Alpha-tubulin antibody

Catalog Number:

2144

The aim of my research is to evaluate effects of novel drugs on Wnt signaling in breast cancer. As I was evaluating protein expression via western blot, I wanted to use an antibody as a loading control. Tubulin was a cheaper alternative to the popularly used actin.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cells

Primary Incubation

1:1000 in 4 degrees overnight

Blocking Agent

5% BSA in TBS-T

Secondary Incubation

Rabbit secondary antibody 1:2000

Tertiary Incubation

N/A

Detection

ECL Reagents

Results Summary

Equal loading by even expression of bands was observed.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to use

The Bad

N/A

The Bottom Line

Great antibody for detecting equal loading of proteins for western blot

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