Excellent Cell Imaging Multi-Mode Reader

University of Texas Medical Branch
Microbiology Immunology
Research Scientist

Overall

Performance

Ease-of-Optimization

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Company:

BioTek Instruments

Product Name:

Cytation 5 Cell Imaging Multi-Mode Reader

Catalog Number:

Cytation 5

This is an excellent all in one piece of table top fit equipment every lab or department should own. It does everything from simple microscopy in brightfield to 60X fluorescent imaging; simple ELISA readings of absorbance to luciferase kinetics. No running from room to room for different types of equipment with reagents in hand or coolers. This is a piece of art. 

Experimental Design and Results Summary

Application

Fluorescence microscopy

Starting Material

HeLa cell culture

Protocol Overview

HeLa cells were fixed and permeabalised according to regular protocols. primary antibody at 1:200 was added overnight and staining with secondary antibody was done at 1:500 and DAPI at 1:2000 for 1h next day. cells were mount in vectashield and dried overnight. next day observations were made at 4X, 20X and 60X

Tips

Be patient while machine focuses for cells.

Results Summary

Excellent publishable data for imaging.

Features Summary

it is all in one machine station

DOI or PMID #

27622505

Additional Notes

N/A

Image Gallery

Summary

The Good

All elaborate features

The Bad

None

The Bottom Line

Buy it!!!

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