Cell Signaling Technology
mTOR (7C10) Rabbit mAb
2983
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mTOR sub cellular localization is very critical for it's activation. mTORC1 activation takes place at the lysosomal surface of cells. During nutrient replete conditions, mTOR is readily observed at the lysosomal surface; however, during depleting conditions, mTOR is diffused throughout the cell. We were interested in observing this phenomenon in T cells during activation. This antibody is strong monoclonal antibody used by the mTOR field with immunofluorescence studies.
Immunofluorescence
Murine CD8 T cells
1:400. Overnight at 4 deg or 2hr at room temperature
5% Goat Serum in PBS (.1% Tween)
Goat anti-Rabbit AF 647 or 488
None
Zeiss LSM780 confocal microscope
We were able to readily identify mTOR localization at the lysosomes in both nutrient replete and deplete conditions. We also observed more localization upon T cell activation.
27064374
N/A
Great monoclonal antibody against mTOR at great cost
None really
Highly recommend this antibody for IF experiments to identify mTOR at the subcellular level.