Cell Applications, Inc.
Rat Astrocytes
R882AK-05n
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We were trying to establish a dose response curve to LPS in order to test anti-inflammatory peptides. However, no amount of LPS treatment was sufficient to activate the astrocytes, making the experiments not possible.
PCR/Western
Cell Applications Rat Astrocytes
Astrocytes were stimulated with concentrations of LPS ranging from 1-10000 ng/mL for 24 hours prior to running PCR/Western
Cells could not be activated increase GFAP expression
All LPS treatements were not significantly different from the control
N/A
Cells were easy to culture and work with
Cells could not be activated
Easy cells to culture; would not use for a study on astrocyte activation as no amount of LPS was able to sufficiently activate the cells.