Staining Of Mouse Bone Marrow Lineage Negative Cells With BV421-CD34

February 26, 2018

Pathology
Northwestern University at Chicago
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

Brilliant Violet 421™ anti-mouse CD34 Antibody

Catalog Number:

562608

BV421-CD34 was used for staining murine bone marrow lineage negative cells for hematopoietic stem/progenitor cells identification combined with other antibodies. The BV421-CD34 can be used at pacific blue channel on FACS Canto II machine.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow lineage negative cells

Primary Incubation

1:100 for 15min at RT

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometer

Results Summary

By flow cytometer assay, the c-Kit and Sac1 double positive cell population (LSK) gated from lineage negative cells can be further distinguished by CD135 and CD34 staining. The long-term hematopoietic stem cells were identified as CD34-CD135-.

DOI or PMID #

N/A

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong staining signal and compatible with Pacific blue channel.

The Bad

None

The Bottom Line

Works well!

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