Antibody To Quantify Cell Surface CD47 Expression

February 22, 2018

Biomedical Engineering
Binghamton University
Research Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Rat Anti-Mouse CD47 Antibody

Catalog Number:

127510

The Alexa Fluor 647 conugated rat anti-mouse CD47 antibody (miap 301) was used to quantify the expression of CD47 on mouse melanoma cells (B16F10 cell line).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

B16F10 mouse melanoma cell line

Primary Incubation

1:100 dilution of 0.2mg/ml antibody for 30 mins at 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

By flow cytometry

Results Summary

The antibody could clearly quantify the cell surface CD47 expressed by B16F10 cells, with optimized flow cytometer settings.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy, efficient, one-step quantification

The Bad

None

The Bottom Line

Great antibody for quick and easy detection of cell surface CD47.

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