Characterizing Polarized T Cells Using T-Bet Monoclonal Antibody

February 20, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

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Company:

Invitrogen Antibodies

Product Name:

T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor 660

Catalog Number:

50-5825-80

T cells were isolated from C57Bl/6 wildtype mice and polarized for 5 days before testing the phenotype via T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor 660 to look for the presence of Th1, Th2, or Th17 transcription factors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 isolated CD4+ T cells

Primary Incubation

0.1ug/1.0x105 cells for 1 hour

Blocking Agent

20ug/ml gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

This antibody works well to investigate lowly expressed transcription factors.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody does not produce high amounts of auto fluorescence

The Bad

None

The Bottom Line

This antibody is relatively inexpensive and serves as a good medium to test for the presence of intracellular transcription factors via flow cytometric analysis.

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