Use Of ROR Gamma (T) Monoclonal Antibody To Characterize T Cell Polarizations

February 16, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

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Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

ROR gamma (t) Monoclonal Antibody (B2D), PerCP-eFluor 710

Catalog Number:

46-6981-80

T cells were isolated from mice and polarized to a Th17 phenotype. This phenotype was then tested via intracellular staining for ROR gamma-T expression after permeabilization.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

T cells from mice

Primary Incubation

0.1ug/1.0x105 cells for 1 hour

Blocking Agent

20ug/ml gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

This antibody works relatively well to look at an intracellular protein, although it does show a large amount of auto fluorescence.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody was easy to calibrate and titer

The Bad

This antibody shows a high amount of auto fluorescence

The Bottom Line

This antibody works relatively well to look at an intracellular protein if the protein of interest is in high quantities within the cell.

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