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Alexa Fluor® 488 anti-mouse/human Helios Antibody
137223
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The goal of this experiment was to assess the expression of Helios in lung CD4+ T cells. This product was selected because of the AF488 tag as it has no spillover into other channels.
Flow Cytometry
Single cell suspension of mouse lung cells
1:200, 30 mins, 4 C
Mouse FcX
None
Blue laser (BD Fortessa)
Antibody worked well. On an average, ~15% of lung CD4 T cells were observed to express Helios.
N/A
Cells were permeabilized with Foxp3 Staining Buffer Set (ebioscience).
Easy to use, great separation
Can be used in multi-color flow cytometry panel with ease.