Biolegend
APC/Cy7 anti-human CD134 (OX40) antibody
350022
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This antibody has been used in a flow cytometry based study immunophenotyping PBMC populations obtained from cancer patients. The results have not yet been published. Multi-color antibody panels were designed to identify a number of lymphocyte populations and tested to ensure appropriate compensation was possible. The expression of a number of different NK cell receptors was examined, with OX40 being one of them.
Flow Cytometry
Human peripheral blood mononuclear cells (PBMC)
5uL APC/Cy7-CD134 for 30 minutes at 4oC
FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)
NA
Beckman Coulter Galios, Kaluza software
Clear staining that allowed the quantification of OX40 expression on NK cells. The antibody fit well into a nine color panel with no compensation issues.
Clear staining and useful for FACS analysis.
The antibody has only been tested for flow cytometry
Easy to use antibody that provided clear and distinct staining of OX40 on an NK cell population.