ECL for Western Blot

The general aim of the experiment is to study the effect of chemoattractant on protein expression. Western blot is a widely used technique to detect the protein in a sample of tissue or cell extracts. The protein was first denatured and then followed by electrophoresis and finally transferred to a membrane and probed for specific protein of interest and finally visualized using ECL solution. This product is very good in detecting excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images by using x-ray film or chemidoc imager.