Clear Detection of CD19+ Lymphocytes

January 14, 2019

Innate Immunity
German Rheumatism Research Center
Graduate Student

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Company:

BioLegend

Product Name:

Brilliant Violet 605 anti-human CD19 Antibody

Catalog Number:

302243

To detect CD19+ B lymphocytes, PBMC were isolated from peripheral blood of a healthy donor. PBMC were stained with viability dye and anti-human CD19 conjugated to BV605 (clone: HIB19; manufacturer: BioLegend)

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC from healthy human donor

Primary Incubation

Dilution 1:50; time 15 min; temp RT

Blocking Agent

Included in in-house staining buffer (PBS + 0.2% BSA)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Directly labelled

Results Summary

Enables clear detection of CD19+ lymphocytes after gating on single viable lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Low titer, bright staining

The Bad

None

The Bottom Line

Recommended!

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