Compensation Control In Flow Cytometric Analysis

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Research Assistant

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Company:

Thermo Fisher Scientific

Product Name:

OneComp eBeads™ Compensation Beads

Catalog Number:

01-1111-42

The OneComp eBeads™ Compensation Beads were used as a single-color compensation controls for the BD LSR II instrument when running flow cytometric analysis.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Compensation Beads

Protocol Overview

One drop of OneComp eBeads™ Compensation Beads was added to a 5ml polypropylene tube with 1 microL of .2 mg/ml or .5mg/ml antibody.

Tips

Do not add more than 1 drop of beads per tube

Results Summary

The compensation beads consistently work well wit the BD LSR II instrument to remove spectral overlap.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

These beads can be used with any type of primary antibody

The Bad

N/A

The Bottom Line

These beads, while relatively expensive, work extremely well to calibrate the instrument and remove the spectral overlap in your flow cytometric experiment.

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