Cell Signaling Technologies
Doublecortin Antibody (DCX)
4604
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This product is used to characterize neurons in different developmental stages. It was specifically chosen to distinguish immature neurons from neural progenitor cells.
Immunofluorescence
Primary Rat Cortical Neurons
1:500 overnight at 4 degrees C in 3% BSA in PBS
10% BSA in PBS
1:2000 with AlexaFluor goat anti-rabbit 488 in 3% BSA in PBS for 45 minutes at room temperature
None
Keyence BZ-9000 Fluorescence Microscope
This antibody contributed to identifying neurons that were developmentally past the neural progenitor stage, but not yet fully mature neurons. This antibody also helped identify neurons that had successfully exited the cell cycle, and can be used to visualize both the cell body and arborizations.
N/A
Requires permeablization
No detected cross-talk with other primary antibodies used in our studies
Not much. Some non-specific signal, but good overall.
Excellent product for immunocytochemistry. Robust signal at a range of concentrations.