Biolegend
APC anti-mouse CD25 Antibody
102011
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I used this antibody to characterize mouse Tregs based on CD25 and Foxp3 expression. I was looking for a cheaper antibody and I found this one from biolegend. This antibody stained well and gave us nice distinct population.
Flow Cytometry
Mouse Thymocytes
1:400 for 3h at 4 degree
0.5% BSA
NA
Flow cytometry
This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.
Cheap and reproducible results
Nothing
Good quality antibody at cheaper price