Antibody Stains CD8b T Cells Very Well

January 23, 2018

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Rat Anti-Mouse CD8b.2

Catalog Number:

553040

We wanted to study the role of CD8+ T cells in a diseased vs healthy state, so we used this antibody to identify CD8+ T cells in situ.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse large intestine

Primary Incubation

1:100 in 1% BSA in PBS overnight

Blocking Agent

5% goat serum in 1% BSA

Secondary Incubation

1:200 in PBS for 1 hour at room temperature

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

The antibody stained CD8+ T cells very well in the intestine

DOI or PMID #

N/A

Additional Notes

There is some non-specific staining seen in the lumen of the crypts in the image

Related Categories

Image Gallery

Summary

The Good

Stains CD8+ T cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying CD8+ T cells via immunofluorescence

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