Invitrogen Antibodies
Occludin Monoclonal Antibody (OC-3F10), FITC
33-1511
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We wanted to examine tight junctions in the gut in diseased vs healthy states, so we used this antibody to visualize the tight junctions between the intestinal epithelial cells
Immunofluorescence
Mouse intestine
1:100 in 1% BSA in PBS overnight
5% goat serum in 1% BSA
1:200 in PBS for 1 hour at room temperature
None
Confocal microscopy
The antibody stains the tight junctions in the intestine very well
N/A
Stains occludin very well
It is a mouse antibody, and it might take a bit of optimization with different fixation methods
The stain works really well, but might need a bit of optimization to get working