Antibody Stains Gamma-Delta T Cells In The Gut Very Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Purified anti-mouse TCR γ/δ Antibody

Catalog Number:

118101

We wanted to examine the localization of gamma-delta T cells in the gut in a diseased vs healthy state, so we used this antibody for immunofluorescence to visualize the cells in situ.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse small intestine

Primary Incubation

1:100 in 1% BSA in PBS overnight

Blocking Agent

5% goat serum in 1% BSA

Secondary Incubation

1:200 in PBS for 1 hour at room temperature

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

The antibody stains gamma-delta T cells in the small intestine very clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Antibody stains gamma-delta T cells very well and specifically

The Bad

None to mention

The Bottom Line

This antibody is great for immunohistochemical visualization of gamma-delta T cells

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