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Pacific Blue™ anti-human IgM Antibody
314514
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Browse Similar Products: IgM Flow Cytometry Antibodies
The general aim of our research is to characterize the phenotype and function of pathogenic B cells in various diseases. We are building fluorescence and mass cytometry panels to study human B cells in the context of autoimmune and idiopathic pulmonary diseases. The main reason I selected IgM-PB was because it fit well in my 14-parameter panel with minimal compensation issues with other antibody conjugates.
Flow Cytometry
Cryopreserved human PBMC
0.1uL (1:2000) IgM-PB in 200uL FACS buffer with 2x10^6 cells; 15 minutes; room temperature
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BD LSRII 5-laser Flow Cytometer
A single color titration of IgM-PB was performed and 0.1uL (1:2000) yielded the highest stain index. We routinely use IgM-PB in our 14-parameter human B cell panel to identify B cells in patient samples with various diseases. Cyropreserved human PBMCs are thawed, washed twice in PBS, and stained with a cocktail of surface antibodies, including IgM-PB. ~2 million cells in 200uL FACS buffer are incubated with antibodies for 15 minutes at room temperature.As evidenced in the attached figure, the staining looks great. It detects the expected frequency of IgM+ B cells in human PBMCs.
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Staining pattern is good, really economical (because you only use at 1:2000!)
Antibody works great!