Biolegend
Zombie UV Fixable Viability Kit
423107
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The aim was to exclude dead cells from the analysis after in vitro culture/stimulation. I chose this product as Biolegend offers quality products at good prices.
Flow cytometry
Human PBMCs
Stain cells (up to 5e5) in 50 ul PBS + Ab cocktail. Add Zombie UV at 1:500 dilution. Incubate for 15 min at RT or 30 min at 4deg. Wash with 150 ul FWB. Proceed to fixation and intracellular staining if needed.
Add Zombie UV dye to the PBS/Ab mix just before staining cells. Avoid any BSA/FCS in the staining medium.
I observed great separation of dead cells, which can be easily excluded from downstream analysis.
NA
Good staining at high dilution
Have to use protein free medium
Great product to achieve live/dead cell discrimination.