Invitrogen Antibodies
Rat IgG1 kappa Isotype Control, eFluor 450
48-4301-80
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In this experiment T cells were extracted from the spleens and lymph nodes of C57B/6 mice using single cell suspensions and negative selection columns, and were then subsequently cultured for 5 days in their respective cytokine milieu's in order to polarize them to a Th1, Th2, or Th17 phenotype. The Rat IgG1 kappa Isotype Control was used to differentiate non-specific background signal from specific antibody signal
Flow Cytometry
C57B/6 CD4+ T cells
0.1ug/1.0x105 cells for 1 hour
20ug/ml gamma globulin
N/A
BD LSRII Flow Cytometer
The Rat IgG1 kappa Isotype Control was very effective in controling for background signal fluorescence.
This control is very easy to use
This is a great control to use at it is easy to optimize and is relatively inexpensive.