Use Of The CD3e Monoclonal Antibody (145-2C11) - PE In The Flow Cytometric Analysis Of CD4+T Cells

January 03, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

CD3e Monoclonal Antibody (145-2C11), PE

Catalog Number:

12-0031-81

This antibody was used in an experiment on CD4+ T cells that were isolated from the spleens and lymph nodes of mice, negatively selected for CD4, and then polarized for 5+ days in a cytokine milieu to induce a particular phenotype, which was then tested utilizing this antibody and flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 isolated CD4+ T cells

Primary Incubation

0.1ug/1.0x105 cells for 30 minutes at 4 degrees celcius

Blocking Agent

Gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

This antibody showed a very consistent signal with little background noise.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This antibody has proven to be consistent over a long period of time

The Bad

Have not found a drawback to this antibody as of yet

The Bottom Line

This is a great antibody to use to detect an extracellular marker because it is relatively inexpensive, gives consistent results over time, and does not require much optimization after titration.

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