The Use Of The Rat IgG2a Kappa Isotype Control, PE-Cyanine5.5 In Flow Cytometry

December 19, 2017

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen Antibodies

Product Name:

Rat IgG2a kappa Isotype Control, PE-Cyanine5.5

Catalog Number:

35-4321-80

The isotype control was utilized in an experiment done to establish the initial phenotype of recently polarized naive CD4+ cells from the spleens and lymph nodes of C57BL/6 male mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 isolated CD4+ T cells

Primary Incubation

0.1ug/1.0x105 cells for 1 hour

Blocking Agent

20ug/ml gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Calibur

Results Summary

This isotype control did in fact help differentiate non-specific background signal from specific antibody signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This control required little to no optomization to garner results

The Bad

N/A

The Bottom Line

This is a fantastic control to use in order to distinguish background signal in your sample.

Share your experience with other scientists. Write a Review! »

Join the discussion