Biolegend
TCR Va24-Ja18 BV711
342922
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To identify iNKT in human PBMC, I tested and titrated the antibody. The goal was to 1. test if the antibody work and 2. to titrate the antibody for downstream experiments. This trial experiment suggests that this antibody can be used to identify iNKT in human PBMCs.
Flow Cytometry
2e6 Human PBMC in FACS buffer
30 min at RT in the dark
NA
BD Fortessa 4
Positive cells identified as iNKT could be detected.
I titrated the antibody by performing seven 2-fold dilution starting at 1:20.
Easy and bright staining
Antibody worked!