PE Anti-Mouse/Human IL-5 Antibody

November 03, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse/human IL-5 Antibody

Catalog Number:

504304

This review is for an IL-5 antibody. I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine single cells suspension

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

1:50 dilution for 1 hour on ice

Tertiary Incubation

None

Detection

FACS

Results Summary

Intracellular levels of IL-5 were detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody for efficient detection of IL-5 on mice immune cells.

The Bad

None

The Bottom Line

Good IL-5 intracellular staining antibody.

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