Bio-Rad
Mouse anti Rat CD31 Clone
MCA1334GA
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The aim is to stain blood vessels in rat skin wounds. It was challenging for us because the tissues we used were fixed by glutaraldehyde. We have tried different antibodies including this one. None of them work.
Immunofluorescence
Rat skin wounds
Overnight at 4C, 1/50-800 dilutions
Goat serum 10%
45min RT
None
Goat anti muse Alexa Fluor® 594
We could not identified stained blood vessels due to extreme background staining. This is a typical issue for glutaraldehyde fixed tissue. Using sodium borohydride failed to reduce the background. If the tissue were fixed by formalin or paraformaldehyde, it might work. Therefore, we don't have a negative review on this antibody.
NA
Not working for glutaraldehyde fixed tissue
Based on our experience, it is not recommended for glutaraldehyde fixed tissue.