BD Biosciences
BD anti-human CD5-BV786
740963
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Browse Similar Products: CD5 Flow Cytometry Antibodies
The general aim of our research is to characterize the phenotype and function of pathogenic B cells in various diseases. We are building fluorescence and mass cytometry panels to study human B cells in the context of autoimmune and idiopathic pulmonary diseases. I selected CD5-BV786 because the UCHT2 is the best CD5 clone and the BV786 channel is a great color option in one of our 14-parameter fluorescence cytometry panels.
Flow Cytometry
Cryopreserved Human PBMCs
0.25uL CD5-BV786 in 200uL FACS buffer with 10^6 cells; 15 minutes; room temperature
None.
N/A
BD LSRII 5-laser Flow Cytometer
A single color titration of CD5-BV786 was performed and 0.25uL CD5-BV786 yielded the highest stain index. We routinely use CD5-BV786 in our 14-parameter human B cell panel to identify CD5+ B cells in patient samples with various diseases. Cyropreserved human PBMCs are thawed, washed twice in PBS, and stained with a cocktail of antibodies including CD5-BV786. 1 million cells in 200uL FACS buffer are incubated with antibodies for 15 minutes at room temperature.As evidenced in the attached figure, the staining looks great: as expected, CD5 is high on T cells and low, but detectable on CD19+ B cells.
NA
Staining pattern is great, good separation
Expensive
It's worth the cost when working with a full (14+ parameter) fluorescence cytometry panel with limited channel options. It's expensive, but you do only need 0.25uL per test.