Proteintech Group Inc
E2F3 Antibody
27615-1-AP
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Browse Similar Products: E2F3 Western Blot Antibodies
We are interested in molecular drivers of cancer, and investigated E2F3 as a potential co-modulator of carcinogenesis. We chose this antibody because it is advertised as being good in both Western blotting and IHC which are the techniques we plan to perform.
Western Blot
Human tumor cell lysates
1:1000 in 5% milk in TBS-T, O/N at 4 degrees C
5% milk in TBS-T, 1 hr, RT
1:10,000 anti-Rb HRP in 5% milk in TBS-T, 30 min, RT
N/A
HRP Chemiluminescence
We observed strong bands at the predicted molecular weight, but also non-specific bands including possibly cross-reaction with actin.
Inexpensive
Non specific bands
Okay for Western, but may not be good for IHC.