PerCP/Cy5.5 Anti-Mouse/Human Helios Antibody For Flow Cytometry

August 31, 2017

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse/human Helios Antibody

Catalog Number:

137229

I am interested in characterizing different subsets of regulatory T cells in the murine colonic lamina propria in the context of colon cancer. Therefore, as a first step we optimized the stain for Helios.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Clear Staining for Helios is observed.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Staining is clear

The Bad

None

The Bottom Line

This is a good antibody

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