Strong Specific and Cheap anti-Mouse Secondary Antibody

September 12, 2017

Biology
Franklin and Marshall College
assistant professor

Overall

Quality of Results

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Company:

Leinco Technologies

Product Name:

Goat Anti-Mouse IgG (H&L) DyLight® 488 Secondary Antibody Adsorbed Against: Hu.

Catalog Number:

M1378

This secondary antibody was used to perform indirect immunofluorescence microscopy on HeLa cells to detect the ER/mitochondria. It showed strong immunoreactivity that was consistent mitochondria. Background levels were low. This antibody is a good choice for immunofluorescent microscopy.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

HeLa cells fixed with 3.7% paraformaldehyde

Primary Incubation

1 hour at room temperature in blocking agent at 1:300

Blocking Agent

PBS in 1% normal goat serum and 0.1% triton x-100

Secondary Incubation

1 hour at room temperature (1:300)

Tertiary Incubation

DAPI

Detection

Confocal microscopy

Results Summary

Great anti-Mouse secondary antibody for half the price!

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific and strong

The Bad

None

The Bottom Line

Inexpensive alternative to Alexa 488!

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