Review Of CD44 Conjugated To Brilliant UV 737

August 25, 2017

Research
Bloodworks NW
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BD Biosciences

Product Name:

BUV737 Rat Anti-Mouse CD44 Clone IM7 (RUO)

Catalog Number:

564392

We wanted to incorporate a second fluorochrome off the UV channel on our LSRII to increase our capacity to 12 colors. We currently use the DAPI channel to detect cell viability using Live/Dead Fixable blue stain and for our second fluorochrome we chose BUV 737.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30 mins@+4C

Blocking Agent

Anti-CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Splenocytes were stained with antibodies against CD4, CD62L and CD44 BUV 737. A CD44 FMO was included to help with gating. Decent separation between CD44 intermediate vs. CD44 hi T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Decent separation between CD44 intermediate and high populations.

The Bad

expensive.

The Bottom Line

Anticipate slightly more optimization than your typical antibody. Using the right filter set (730/45) for this fluorochrome is absolutely key to minimizing spill over into the Alexa700 channel.

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