Cyclin D1 Antibody For Western Blotting

August 28, 2017

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Cyclin D1 Rabbit mAb

Catalog Number:

2978

The antibody is specific for Cyclin D1 and is user-friendly. No optimization is necessary for applying this antibody to western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human ovarian cancer cells

Primary Incubation

1:1000 dilution overnight at 4 degrees on shaker

Blocking Agent

5% BSA in TBS-T

Secondary Incubation

1:2000 Rabbit secondary (BioRad), 1 hour incubation at room temperature on shaker

Tertiary Incubation

N/A

Detection

Pierce ECL Western Blotting Substrate

Results Summary

Compared to the first lane (untreated), the other three treatments reduced Cyclin D1 expression in ovarian cancer cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Antibody does not show much nonspecificity

The Bad

N/A

The Bottom Line

Great antibody for Cyclin D1 in western blotting.

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