Survivin Antibody For Western Blotting

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Survivin Rabbit mAb

Catalog Number:

2808

This is a great antibody for detecting survivin by western blotting. Non-specific bands are not evident.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human ovarian cancer cells

Primary Incubation

1:1000 dilution overnight at 4 degrees on shaker

Blocking Agent

5% BSA in TBS-T

Secondary Incubation

1:2000 Rabbit secondary (BioRad), 1 hour incubation at room temperature on shaker

Tertiary Incubation

N/A

Detection

Pierce ECL Western Blotting Substrate

Results Summary

Compared to first band (untreated), survivin expression of the cells with three different treatments was reduced.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Highly specific antibody

The Bad

N/A

The Bottom Line

The antibody from Cell Signaling is great for detecting survivin expression with minimal nonspecificity.

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