BioXCell
ReadyTag anti-GFP
RT0265
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This antibody was used to detect over-expressed fluorescent proteins transfected in 293T cells via immunoblot. The antibody was very specific for A. victoria-derived fluorescent proteins but not coral-derived fluorescent proteins.
Western Blot
Transfected 293T cells
2 hours shaking at 1:5000 at room temperature in blocking agent
5% milk in TBST
Goat anti-mouse (Santa Cruz Biotechnology) 1 hour shaking at 1:10,000
None
Western bright ECL
This antibody detected several A. victoria-derived fluorescent proteins, including EGFP, mCerulean, MT-Sapphire and mCitrine. It was not able to detect fluorescent proteins from Coral ssp (Discosoma, Galaxae and Fungia). All fluorescent proteins were visible under confocal microscopy. Two non-specific bands were seen: 120kDa and 25kDa, but these were minor.
N/A
Great price for a great antibody
Smallest size is 1mg
This antibody is comparable to JL-8 from Clontech, but for far less money.