SIGMA-ALDRICH
Anti-ARG1 (AB2) rabbit antibody
AV45673
Browse Similar Products: Arginase 1 Western Blot Antibodies
Analysis of arginase-1 has become an important tool in tumor immunology since high arginase levels are believed to be associated with tumor-promoting activity of tumor-associated leukocytes. We analyzed the levels of arginase in murine tumor-assoaciated macrophages in comparison with bone marrow-derived macrophages polarized either to M1 (tumor-suppressing) or M2 (tumor-promoting) phenotype. We used the anti-ARG1 rabbit antibody from Aldrich to prove the M2 phenotype of LLC tumor-associated murine macrophages.
Western Blot
Lysates of murine macrophages
1 ug/ml in PBS supplemented with 5% nonfat milk and 0.05% Tween 20; overnight, cold room
5% nonfat milk in PBS/0.05% Tween 20
goat anti-rabbit IgG conjugated with HRP; 1:3,000; 1 hr, room temperature
None
West Pico
This antibody correctly demonstrates the expression of arginase-1 in distinct types of murine macrophages, namely almost negative or very ow levels in M1 macrophages (left lane), positive expression in M2 macrophages (middle lane) and high levels of expression in tumor-associated macrophages (right lane).
N/A
The antibody also detects arginase in human cells.
High sensitivity and specificity
None detected
This antibody is an excellent tool to detect by western blotting arginase-1 in murine and also in human cells.