Biolegend
FITC anti-human CD66b Antibody
305103
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Browse Similar Products: CD66b Flow Cytometry Antibodies
The goal was to classify the granulocyte activation status in whole blood of patients with different inflammatory disorders (chronic vs. akute). Therefore, different activation markers were combined in a single panel. This antibody was chosen because the concentration most suitable for this sample material is known.
Flow Cytometry
Human Whole Blood
10 Mins at Room temperature with blocking agent (5µl/100µl)
Tru Stain FcX (BioLegend)
5µl antibody/100µl whole blood, 30 Mins on ice in the dark
15 Mins on ice in the dark with BD Blood Lysing Solution, wash 2 times with FACS-Buffer (PBS with 1%BSA)
Good antibody with stable and easily detectable/classifiable signal. No unspecific staining. Be sure to block with Tru Stain Blocking reagent or another blocking agent prior to staining. Be sure to stain with antibody before lysing Erythrocytes
N/A
Easy to use, reliable, no background signal or unspecific staining
Great for quick whole blood staining as well as monitoring PBMC purity.