Beads For Isolation Of Hematopoietic Stem Cells From PBMCs

Albert Einstein College of Medicine
Genetics
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Company:

Miltenyi Biotec

Product Name:

CD34 MicroBead Kit, human

Catalog Number:

130-046-702

Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. One of the models we have generated consists of injecting human hematopoietic stem cells into immunocompromised NSG mice. We later infect these mice with HIV to study different aspects of infection in vivo. We use this kit to purify human hematopoietic stem cells from blood.

Experimental Design and Results Summary

Application

Cell purification

Starting Material

Human PBMCs

Protocol Overview

Follow manufacturers protocol

Tips

Titrate the beads as you might be able to use less than the protocol states

Results Summary

Good purity, yield and viability of CD34+ cells after purification

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works well

The Bad

Expensive

The Bottom Line

Great kit for isolation of human stem cells

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