R&D Systems
Recombinant Human VEGF 165 Protein, CF
293-VE-010/CF
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VEGFA induces human retinal endothelial cell migration. VEGFA was used at a concentration of 40 ng/ml and the migration of human retinal endothelial cells was measured by a wound healing assay.
Cell migration assay
Human retinal endothelial cells
Cells were seeded in IBIDI chambers and once the cells reach near to confluent, the cells were quiesced for overnight. The cells were treated with VEGFA and the migration of the cells was measured as a percent of wound closure.
The endothelial cells tend to detach while washing so care must be taken while changing the media after quiescing the cells.
VEGFA induces human retinal endothelial cells.
N/A
This is a best agonist to study the mechanisms of angiogenesis.
Little expensive
VEGFA is a potent mediator of angiogenesis and it induces various signaling molecules involved in angiogenesis!