Good Antibody To Detect Macrophage Mannose Receptor (MMR, CD206) On Mature Human Macrophages

July 19, 2017

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor 488 anti-human CD206 (MMR) Antibody

Catalog Number:

321113

The mouse mAb 15-2 conjugated with Alexa Fluor 488 from BioLegend is a nice tool to detect mature human macrophages expressing MMR (CD206).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human macrophages maturated in vitro from periperal blood monocytes

Primary Incubation

1:50 dilution in PBS/2% BSA

Blocking Agent

2% BSA in PBS

Secondary Incubation

NucBlue to highlight cell nuclei

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

The antibody specifically stains mature human macrophages polarized towards M2 phenotype (expressing MMR) and does not stain monocytes and M1-polarized macrophages that are known to be MMR-negative.

DOI or PMID #

N/A

Additional Notes

Image shows positive staining of human macrophages polarized towards M2 phenotype (left) and mainly negative staining of M1-macrophages (right).

Related Categories

Image Gallery

Summary

The Good

The antibody is easy to use in IF applications.

The Bad

The antibody should be carefully titrated to avoid high background levels.

The Bottom Line

This is a good directly conjugated antibody allowing to discriminate mature M2-macrophages from M1-macrophages or monocytes.

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