R&D Systems
Goat Anti-Chicken IgY Antibody
AF010
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I have used a primary antibody that was generated in Chicken for Western blot application. It is not so common to get an anti-Chicken antibody conjugated with HRP, so I have used Goat anti-Chicken antibody as a secondary antibody and bovine anti-goat conjugated with HRP as a tertiary antibody. It worked perfectly!
Western Blot
Human plasma
Anti-human AFP antibody
5% skim milk powder
Goat anti-Chicken IgY unconjugated
Bovine anti-goat IgG HRP
Chemiluminescence
The AFP levels were measured by Western blot analysis.
N/A
Very good antibody and easy to optimize
Little background!
Very good antibody to try if the primary antibody is raised in chicken!