Miltenyi Biotec
CD34 MicroBead Kit UltraPure. human
130-100-453
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We use CD34 as a marker gene in our retroviral vector used for the transduction of T cells. We use CD34 because it is not expressed on T cells and we can use reagents to purify our transduced populations and visualize them via flow using CD34 antibody staining. We use these CD34 immuno magnetic beads to purify our T cell populations to only include transduced T cells after the transduction process.
Purification
Human T cells
You incubate your cells with these beads for 30 mins. Wash the cells and run them through a column attached to a magnet. Flow through will be unlabeled cells.
Don't try too purify too many cells, columns will run slowly.
After CD34 enrichment, there is a definite more pure CD34 population, where the CD34 negative cells have been washed out.
N/A
High enrichment
You lose quite a lot of cells through purification and the yield is always less than calculated.
CD34 immuno beads are useful to purify even small CD34 positive populations.