gentleMACS Dissociator For Suspending Tough Tissues

The Gladstone Institutes
GIVI
Research Associate

Overall

Performance

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

gentleMACS Dissociator

Catalog Number:

130-093-235

My lab studies CD8 T-cell mediated immunity in the genital mucosa and so we utilize this dissociator to break up evolutionarily tough tissues to free and suspend the cells inside.

Experimental Design and Results Summary

Application

Dissociating immune cells from mucosal tissues

Starting Material

Mice mucosal tissues

Protocol Overview

Mice tissues undergo various steps of enzymatic digestion before being placed inside gentleMACS tubes for further mechanical dissociation on this instrument.

Tips

The speed at which the machine runs is extremely important

Results Summary

Results derived from the use of this instrument are reliable. This instrument has become crucial for the work we do in lab to isolate lymphocytes.

Features Summary

Flexible adjustable speeds

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to setup and easy to use

The Bad

The tubes that work with this instrument are extremely expensive

The Bottom Line

Good machine for dissociation. Tubes may be reused.

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