Invitrogen Antibodies by Thermo Fisher Scientific
F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594
A-11020
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The aim is to stain human gingival epithelial cell tight junction molecule, JAM-A, using immunofluorescence. The result can be seen in both frozen section and paraffin embedded sections
Immunofluorescence
Human gingival epithelial tissue
4C overnight with mouse anti-human JAM-A (Santa Cruz Biotechnology, SC-53623)
10% goat serum with 1%BSA in PBS
1/1000 F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594
None
Fluorescence
Good staining with little background
N/A
It works well in both frozen and paraffin sections.