Atlas Antibodies
Anti-Cep128 Antibody
HPA001116
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Mature centrioles have assessor structures (called distal and subdistal appendages) attached near their distal ends. My work attempts to elucidate the function of the subdistal appendages of the centriole. We double checked the localization of Cep128 and centriolin to confirm that they localize to the subdistal appendages. We also needed these reliable antibodies to confirm depletion of the proteins with RNAi and CRISPR. The antibodies also allowed us to determine which conditions lead to the mislocalization of these proteins (Cep128, Centriolin, ODF2, ninein, and more).
Immunofluorescence
Cultured human and mouse cell lines
1:500 dilution
3% BS, 0.1% triton in PBS
Goat anti-Rabbit 594 antibody (molecular probes)
None
Flourescent microscopy
Excellent staining in human cells. Hela and RPE-1weak staining in mouse derived cell lines. 3T3, IMCD3
27875681
N/A
It works well in human
Weak staining in mouse cells
Reliable antibody for immunofluorescence.