BD Biosciences
Anti-human CD8 BUV805
564912
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One of our lab focuses is studying lymphocytes in various human tissue sites. We extracted lymphocytes from different human spleen samples and wanted to compare marker expression. We need antibody that will clearly identify these CD8+ T cells. We chose this antibody because it is dim and thus will not cause a lot of spill over on this high population.
Flow Cytometry
Human spleen lymphocytes
Cells were stained in 100uL FACS buffer with antibody at 1:50; for 20 minutes on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
LSR II
We were able to successfully mark and identify CD8+ lymphocytes in spleen lung tissue cells and distinguish them from CD8+ cells.
N/A
Low spillover with large populations
Good antibody for samples with high number of CD8+ cells.